appendix S1: DNA extraction and sequencing protocol. appendix S2: Species-delimitation method. appendix S3: Integrative delimitations: fig. S1. Integrative delimitations for an Oreophryne and Cornufer clade. fig. S2. Integrative delimitations for four Oreophryne clades. fig. S3. Integrative delimitations for a Xenorhina and Oreophryne clade. fig. S4. Integrative delimitations for two Cornufer clades. fig. S5. Integrative delimitations for a Hylophorbus and Nyctimystes clade. fig. S6. Integrative delimitations for a Choerophryne and Oreophryne clade. Appendix S4: Subregions choice. fig. S7. Distribution of GenBank mitochondrial DNA sequences, per locus. fig. S8. Ultrametric tree of amphibians from New Guinea and neighbouring islands (16S rRNA). fig. S9. Endemism rate of amphibian species based on the different datasets. table S1. Summary of the acoustic data analysed in appendix S3. table S2. Summary of the number of total mitochondrial DNA sequences, recognised species and candidate species. table S3. List of available mitochondrial DNA sequences for amphibians of the New Guinea region, per species. table S4. Congruence matrix between our different species-delimitation methods. table S5. Congruence matrix between different species-delimitation methods, for the 16S and COI locus. (.docx)