Research Article
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Article title
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Abstract
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Keywords
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Introduction
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Materials and methods
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Study sites
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Sampling approach
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Environmental variables
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Soil physical and chemical analyses
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Monthly bioclimatic variables
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Transformation and selection of environmental variables
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DNA extraction, V4 18S rRNA amplification, and Illumina sequencing
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Sequence processing, taxonomic assignment, and tag-jump control
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Diversity analyses
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Taxonomic composition and alpha diversity: whole soil protist community
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Beta diversity: whole soil protist community
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Diversity analyses for four major protist groups
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Results
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Metabarcoding of soil protists’ environmental DNA
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Taxonomic composition, and alpha diversity
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Spatial and temporal beta diversity in soil protist assemblages
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Inter-seasonal variations in soil protist assemblages
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Edaphic predictors of soil protist community beta diversity
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Alpha and beta diversity patterns of four major protist groups
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Discussion
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Conclusion
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Acknowledgments
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Author contributions
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Data Accessibility Statement
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References
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Supplementary material
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